CD30 expression identifies a functional alloreactive human T-lymphocyte subset.
Transplantation, 1998/5/15;65(9):1240-7.
Martinez OM[1], Villanueva J, Abtahi S, Beatty PR, Esquivel CO, Krams SM
Affiliations
PMID: 9603174
Impact factor: 5.385
Abstract
background: CD30 is a member of the tumor necrosis factor/nerve growth factor receptor family and has been proposed as a marker of specific cytokine-producing subsets in humans. Previous studies have examined the expression of CD30 on established T helper type 1 and T helper type 2 cell clones and the function of CD30+ cells after mitogenic stimulation. In this study, we examined the development and function of CD30+ T cells generated in response to alloantigen.
methods: Primary one-way mixed lymphocyte reactions were established, and the expression of CD30 on T lymphocytes was determined by immunofluorescence and flow cytometry. Fluorescence-activated cell sorting was utilized to define the cytokine profile of alloactivated CD30+ cells after restimulation with anti-CD3 monoclonal antibodies or alloantigen. The effect of cyclosporine on the development of CD30+ cells, and on cytokines produced by CD30+ T lymphocytes, in response to alloantigen was determined.
results: CD30+ T lymphocytes could be detected on day 2 of mixed lymphocyte reactions and continued to increase in number and proportion through day 6. Both CD4 and CD8 T cells expressed CD30 after primary alloantigenic stimulation. CD30+ T cells are a subset of alloactivated T cells and are the major source of interferon-gamma and interleukin-5 produced in response to alloantigen. Cyclosporine partially, but not completely, inhibits the development of CD30+ cells, and has a greater effect on interferon-gamma production than on interleukin-5 production.
conclusions: CD30+ T lymphocytes may constitute an important immunoregulatory subset in human allograft rejection.
MeSH terms
CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cyclosporine; Cytokines; Humans; Immunosuppressive Agents; Interferon-gamma; Interleukin-2; Interleukin-5; Isoantigens; Ki-1 Antigen; Kinetics; T-Lymphocyte Subsets
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