Combinational variation of restriction modification specificities in Lactococcus lactis.
Mol Microbiol, 1998/4;28(1):169-78.
Schouler C[1], Gautier M, Ehrlich SD, Chopin MC
Affiliations
PMID: 9593305
Impact factor: 3.979
Abstract
Three genes coding for a type I R-M system related to the class C enzymes have been identified on the chromosome of Lactococcus lactis strain IL1403. In addition, plasmids were found that encode only the HsdS subunit that directs R-M specificity. The presence of these plasmids in IL1403 conferred a new R-M phenotype on the host, indicating that the plasmid-encoded HsdS is able to interact with the chromosomally encoded HsdR and HsdM subunits. Such combinational variation of type I R-M systems may facilitate the evolution of their specificity and thus reinforce bacterial resistance against invasive foreign unmethylated DNA.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; Bacteriophages; Cloning, Molecular; DNA Restriction-Modification Enzymes; Gene Amplification; Genes, Bacterial; Lactococcus lactis; Molecular Sequence Data; Mutagenesis, Site-Directed; Polymerase Chain Reaction; Sequence Analysis, DNA
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