Isolation and characterization of a plasmid from Lactobacillus fermentum conferring erythromycin resistance.
Plasmid, 1997;37(3):199-203.
Fons M[1], Hégé T, Ladiré M, Raibaud P, Ducluzeau R, Maguin E
Affiliations
PMID: 9200223
Impact factor: 3.085
Abstract
Lactobacillus fermentum is a lactic acid bacterial species commonly found in the digestive tracts of pigs and rodents and also present in man. We characterized a 5.7-kb plasmid, pLEM3, conferring erythromycin resistance, which was isolated from a porcine strain of L. fermentum. Plasmid pLEM3 established efficiently in L. fermentum, conferred high-level erythromycin resistance (MIC > 1 mg/ml), and was segregationally stable. A deletion derivative of pLEM3, called pLEM5, was constructed and found to be as genetically stable as the parent. A multiple cloning site was inserted into pLEM5, generating plasmid pLEM7. Nucleotide sequence determination of pLEM5 revealed similarities with known genes. The replicon itself is a member of the pC194 family of rolling circle plasmids. The region responsible for erythromycin resistance was 98.2% identical to the erm gene of conjugative transposon Tn1545.
MeSH terms
Animals; Cloning, Molecular; Drug Resistance, Microbial; Erythromycin; Lactobacillus; Molecular Sequence Data; Plasmids; Sequence Analysis, DNA; Sequence Homology; Swine
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