Molecular properties of the erythromycin resistance plasmid pPV141 from Staphylococcus chromogenes.
Plasmid, 1997;37(2):119-27.
Somkuti GA[1], Solaiman DK, Steinberg DH
Affiliations
PMID: 9169203
Impact factor: 3.085
Abstract
The 2.3-kb erythromycin resistance (EmR) plasmid pPV141 of Staphylococcus chromogenes 3688 was isolated and characterized. Nucleotide sequence analysis identified ORF1 and ORF2 separated by a 445-bp spacing, encoding a 158-residue replication protein (Rep141) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respectively. Structural analysis and Southern hybridization showed that the rep and ermM genes in pPV141 shared homology with other known EmR plasmids. Based on sequence analysis, pPV141 was classified as a unique member of the pSN2 family of EmR plasmids.
MeSH terms
Amino Acid Sequence; Anti-Bacterial Agents; Bacterial Proteins; Base Sequence; DNA Helicases; DNA Replication; DNA, Bacterial; DNA-Binding Proteins; Drug Resistance, Microbial; Erythromycin; Genes, Bacterial; Methyltransferases; Molecular Sequence Data; Nucleic Acid Conformation; R Factors; Restriction Mapping; Sequence Homology, Nucleic Acid; Staphylococcus; Thermodynamics; Trans-Activators
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