The sequence of the mer operon of pMER327/419 and transposon ends of pMER327/419, 330 and 05.
Gene, 1994/8/19;146(1):73-8.
Hobman J[1], Kholodii G, Nikiforov V, Ritchie DA, Strike P, Yurieva O
Affiliations
PMID: 8063107
Impact factor: 3.913
Abstract
Three different, independently isolated mercury-resistance-conferring plasmids, pMER327/419, pMER330 and pMER05, from cultures originating from the river Mersey (UK), contain identical regulatory merR genes and transposon ends. The mer determinant from pMER327/419 contains an additional potential ORF (ORF F) located between merP and merA when compared with the archetypal Tn501. Although these plasmids confer narrow-spectrum resistance (resistance to Hg2+, but not organomercurials) their merR genes encode a potential organomercurial-sensing protein. Transposition of the mer of pMER05 into plasmid RP4 was demonstrated and, as with Tn502 and Tn5053, insertion occurred at a specific region. The sequence of pMER05 is identical at the 'left' and 'right' termini and across merR to Tn5053, which was independently isolated from the chromosome of a Xanthomonas sp. bacteria from the Khaidarkan mercury mine in Kirgizia, former Soviet Union [Kholodii et al., J. Mol. Biol. 230 (1993a) 1103-1107]. The transpositional unit of pMER05 is, like that of Tn5053, bounded by DNA homologous to the imperfect 25-bp inverted repeats (IR) of the In2 integron, which brackets antibiotic-resistance cassettes in Tn21 subgroup transposons. At one end of the transposable element, and internal to the In2-like IR, is a 38-bp IR which closely resembles the IR that bounds Tn21.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA Transposable Elements; DNA-Binding Proteins; Genes, Bacterial; Molecular Sequence Data; Open Reading Frames; Operon; Plasmids
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