Two identical copies of IS1246, a 1275 base pair sequence related to other bacterial insertion sequences, enclose the xyl genes on TOL plasmid pWW0.

Microbiology (Reading), 1994/9;140 ( Pt 9):2305-7.

Reddy BR[1], Shaw LE, Sayers JR, Williams PA

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PMID: 7952183

Impact factor: 2.956

Abstract
Two identical direct repeats of a 1275 bp sequence, designated IS1246, encompass the xyl genes, which determine the catabolism of toluene, m- and p-xylenes to central metabolites, on the TOL catabolic plasmid pWW0. IS1246 has a terminal inverted repeat of 12 bp (5'GGGCACCTCGAA3') and contains a major open reading frame of 280 codons. This ORF shows significant homology with ORFs encoded by a number of bacterial insertion sequences from Bacteroides, Neisseria and Escherichia coli.
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