Cloning and sequence analysis of a plasmid-encoded 2-haloacid dehalogenase gene from Pseudomonas putida No. 109.
Biosci Biotechnol Biochem, 1994/1;58(1):160-3.
Kawasaki H[1], Toyama T, Maeda T, Nishino H, Tonomura K
Affiliations
PMID: 7764511
Impact factor: 2.337
Abstract
The 2-haloacid dehalogenase of Pseudomonas putida No. 109 was mediated by a 74-kb conjugative plasmid, which was transferred by mating into Pseudomonas and Escherichia coli and there expressed the dehalogenase. A 2.8-kb EcoRI-fragment generated from the plasmid was cloned and sequenced. The dehalogenase gene (dehH109) was identified by comparison with the N-terminal amino acid sequence and the molecular weight of the enzyme protein. The gene dehH109 coded for a 224-amino acid protein of M(r) 25,231, which showed significant homology to the other four L-specific 2-haloacid dehalogenases from Pseudomonas sp. CBS3, P. putida AJ1, and Xanthobacter autotrophicus GJ10 and also to the haloacetate dehalogenase H-2 from Moraxella sp. strain B, but no homology with another haloacetate dehalogenase H-1 and the D-specific 2-haloacid dehalogenase from P. putida AJ1.
MeSH terms
Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Bacterial; Escherichia coli; Gene Deletion; Hydrolases; Molecular Sequence Data; Plasmids; Pseudomonas putida; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid
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