The nucleotide sequence of a transposable haloalkanoic acid dehalogenase regulatory gene (dehRI) from Pseudomonas putida strain PP3 and its relationship with sigma 54-dependent activators.
Biodegradation, 1995/9;6(3):247-55.
Topping AW[1], Thomas AW, Slater JH, Weightman AJ
Affiliations
PMID: 7579999
Impact factor: 3.731
Abstract
The mobile genetic element, DEH found in Pseudomonas putida PP3 carries a 2-haloalkanoic acid dehalogenase structural gene, dehI, and its associated regulatory gene, dehRI. The nucleotide sequence of dehRI was determined. The gene had an open reading frame putatively encoding for a 64 kDa protein containing 571 amino acid residues. The protein was similar to previously published sequences of several other sigma 54-dependent activator proteins. Amino acid sequence analysis showed that the deduced DehRI protein clustered with the NifA nitrogenase regulatory activator family, and was most closely related, with 47.7% similarity, to a 'NifA-like' deduced partial sequence from a plasmid-encoded ORF in Pseudomonas sp. strain NS671, associated with L-amino acid production. The domain structure of DehRI was analysed by alignment with other NifA-like and NtrC-like sequences and showed a highly conserved central region of approximately 230 amino acids, and a potential DNA-binding domain. No homology was detected between the deduced DehRI and other sigma 54-dependent activator sequences at the N-terminus, a result which was consistent with that region being the domain which recognised inducer.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA Transposable Elements; DNA-Binding Proteins; DNA-Directed RNA Polymerases; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Hydrolases; Molecular Sequence Data; Open Reading Frames; Pseudomonas putida; RNA Polymerase Sigma 54; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sigma Factor; Transcription Factors
More resources
EndNote: Download