Alkylation of protein by methyl methanesulfonate and 1-methyl-1-nitrosourea in vitro.
Cancer Lett, 1984/5;23(1):9-17.
Paik WK, DiMaria P, Kim S, Magee PN, Lotlikar PD
PMID: 6331636
Impact factor: 9.756
Abstract
Methylation of horse heart cytochrome c has been examined in vitro with [methyl-14C]methanesulfonate (MMS) and [1-methyl-14C]-1-nitrosourea (MNU) as alkylating agents. Analysis of protein hydrolyzates by an automatic amino acid analyzer indicates that, at pH 9.0 with MMS, epsilon-N-monomethyl-lysine is found to be the only major methylated basic amino acid. On the other hand, the identity of the predominant basic amino acid residue which is [methyl-14C]-labeled by MNU cannot be determined at present. Peptide mapping of chymotryptic digests of cytochrome c after reaction with MMS reveals a lack of specificity in methylation of a specific lysine residue in this hemoprotein.
MeSH terms
Alkylating Agents; Amino Acids; Chymotrypsin; Cytochrome c Group; Histone-Lysine N-Methyltransferase; Hydrogen-Ion Concentration; In Vitro Techniques; Methyl Methanesulfonate; Methylation; Methylnitrosourea; Nitrosourea Compounds; Peptides; Protein Methyltransferases; Proteins
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