Two odorant receptors regulate 1-octen-3-ol induced oviposition behavior in the oriental fruit fly.
Commun Biol, 2023/02/15;6(1):176.
Xu L[1, 2], Jiang HB[1, 2], Yu JL[1, 2], Pan D[1, 2], Tao Y[1, 2], Lei Q[1, 2], Chen Y[1, 2], Liu Z[1, 2], Wang JJ[3, 4]
Affiliations
PMID: 36792777DOI: 10.1038/s42003-023-04551-5
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Abstract
The oriental fruit fly Bactrocera dorsalis (Hendel) is a notorious pest of fruit crops. Gravid females locate suitable oviposition sites by detecting host plant volatiles. Here, we demonstrate that 1-octen-3-ol, a volatile from mango, guides the oviposition behavior of female flies. Two odorant receptors (BdorOR7a-6 and BdorOR13a) are identified as key receptors for 1-octen-3-ol perception by qPCR analysis, heterologous expression in Xenopus laevis oocytes and HEK 293 cells followed by in vitro binding assays, as well as CRISPR/Cas9 genome editing in B. dorsalis. Molecular docking and site-directed mutagenesis are used to determine major binding sites for 1-octen-3-ol. Our results demonstrate the potential of 1-octen-3-ol to attract gravid females and molecular mechanism of its perception in B. dorsalis. BdorOR7a-6 and BdorOR13a can therefore be used as molecular targets for the development of female attractants. Furthermore, our site-directed mutagenesis data will facilitate the chemical engineering of 1-octen-3-ol to generate more efficient attractants.
MeSH terms
Humans; Female; Animals; Receptors, Odorant; Oviposition; HEK293 Cells; Molecular Docking Simulation; Drosophila; Octanols
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