Limosilactobacillus walteri sp. nov., a novel probiotic antimicrobial lipopeptide-producing bacterium.

FEMS Microbiol Lett, 2023/01/17;370

Saroha T[1, 2], Sharma S[1], Choksket S[3], Korpole S[3], Patil PB[1]

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PMID: 36646427DOI: 10.1093/femsle/fnad004

Impact factor: 2.82

Abstract
A Gram-positive facultative anaerobe, nonspore forming, and nonmotile bacterial strain M31 was isolated from faecal contaminated soil. The strain is previously reported to produce a novel antimicrobial lipopeptide and displayed probiotic properties. The strain M31 is catalase negative and fermented d-galactose, d-glucose, esculin, d-maltose, d-lactose, d-melibiose, d-raffinose, d-saccharose (weak reaction), d-xylose (weak reaction), d-ribose (weak reaction), and l-arabinose (weak reaction). The majority of fatty acids were C16:0 (53.9%), C18:0 (26.9%), and C19:0 cyclo ω8c (19.1%). The genome is 2 234 040 bp long with 38.81% guanine-cytosine (GC) content. The pairwise ortho average nucleotide identity and digital DNA-DNA hybridization values of strain M31 with its closest relative species from Limosilactobacillus reuteri clade and Lm. rudii is below the recommended cut-off of 95% and 70%, respectively. Herein, we propose Lm. walteri sp. nov. as a novel species of the genus Limosilactobacillus with M31 = MTCC 12838 = JCM 32759 = KCTC 25569.

Keywords: Limosilactobacillus walteri sp. nov; bacteria; genome; phylogeny; probiotic; taxonogenomics

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