Single-cell transcriptome atlas reveals protective characteristics of COVID-19 mRNA vaccine.
J Med Virol, 2023/01;95(1):e28161.
Tan Y[1, 2], Lu S[3], Wang B[1], Duan X[1], Zhang Y[4, 5], Peng X[3], Li H[6], Lin A[7], Zhan Z[1, 2], Liu X[4, 5]
Affiliations
PMID: 36124363DOI: 10.1002/jmv.28161
Impact factor: 20.693
Abstract
Messenger RNA (mRNA) vaccines are promising alternatives to conventional vaccines in many aspects. We previously developed a lipopolyplex (LPP)-based mRNA vaccine (SW0123) that demonstrated robust immunogenicity and strong protective capacity against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in mice and rhesus macaques. However, the immune profiles and mechanisms of pulmonary protection induced by SW0123 remain unclear. Through high-resolution single-cell analysis, we found that SW0123 vaccination effectively suppressed SARS-CoV-2-induced inflammatory responses by inhibiting the recruitment of proinflammatory macrophages and increasing the frequency of polymorphonuclear myeloid-derived suppressor cells. In addition, the apoptotic process in both lung epithelial and endothelial cells was significantly inhibited, which was proposed to be one major mechanism contributing to vaccine-induced lung protection. Cell-cell interaction in the lung compartment was also altered by vaccination. These data collectively unravel the mechanisms by which the SW0123 protects against lung damage caused by SARS-CoV-2 infection.
Keywords: SARS-CoV-2; inflammatory responses; lung protection; mRNA vaccine; polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs)
MeSH terms
Humans; Animals; Mice; COVID-19 Vaccines; COVID-19; SARS-CoV-2; Viral Vaccines; RNA, Messenger; Macaca mulatta; Endothelial Cells; Transcriptome; Vaccination; Antibodies, Neutralizing; Antibodies, Viral; Spike Glycoprotein, Coronavirus; Immunogenicity, Vaccine
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download