Cross-reaction of naturally-produced β-lactamases from Citrobacter farmeri and Citrobacter amalonaticus with immunological detection of CTX-M enzymes.

Diagn Microbiol Infect Dis, 2022/10;104(2):115760.

Ortiz de la Rosa JM[1], Bouvier M[2], Poirel L[3], Greub G[4], Blanc D[5], Nordmann P[6]

Affiliations

PMID: 35940102DOI: 10.1016/j.diagmicrobio.2022.115760

Impact factor: 2.983

Abstract
The NG-Test CTX-M MULTI immunochromatographic assay has been developed to identify CTX-M-type β-lactamases in Enterobacterales, being the most widespread extended-spectrum β-lactamases. We showed here that the chromosomally-encoded ß-lactamases from Citrobacter farmeri and Citrobacter amalonaticus generated false-positive NG-Test CTX-M MULTI results, compromising the specificity of the test.

Keywords: CdiA β-lactamases; Immunochromatographic assay; NG-Test CTX-M MULTI

MeSH terms
More resources
EndNote: Download