Overdominant expression of related genes of ion homeostasis improves K+ content advantage in hybrid tobacco leaves.
BMC Plant Biol, 2022/7/12;22(1):335.
Pi K[1, 2], Luo W[1, 2], Mo Z[1, 2, 3], Duan L[1, 2, 3], Ke Y[1, 2], Wang P[1, 2, 3], Zeng S[1, 2], Huang Y[4, 5], Liu R[6, 7]
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PMID: 35820807DOI: 10.1186/s12870-022-03719-1
Impact factor: 5.26
Abstract
background: Potassium(K+) plays a vital role in improving the quality of tobacco leaves. However, how to improve the potassium content of tobacco leaves has always been a difficult problem in tobacco planting. K+ content in tobacco hybrid is characterized by heterosis, which can improve the quality of tobacco leaves, but its underlying molecular genetic mechanisms remain unclear.
results: Through a two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 with weak heterosis were screened out. Transcriptome analyses revealed that 80.89% and 57.28% of the differentially expressed genes (DEGs) in the strong and weak heterosis combinations exhibited an overdominant expression pattern, respectively. The genes that up-regulated the overdominant expression in the strong heterosis hybrids were significantly enriched in the ion homeostasis. Genes involved in K+ transport (KAT1/2, GORK, AKT2, and KEA3), activity regulation complex (CBL-CIPK5/6), and vacuole (TPKs) genes were overdominant expressed in strong heterosis hybrids, which contributed to K+ homeostasis and heterosis in tobacco leaves.
conclusions: K+ homeostasis and accumulation in tobacco hybrids were collectively improved. The overdominant expression of K+ transport and homeostasis-related genes conducted a crucial role in the heterosis of K+ content in tobacco leaves.
Keywords: Heterosis; Ion homeostasis; Potassium; Tobacco
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