Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis.
BMC Genomics, 2021/11/17;22(1):829.
Liu Y[1], Han R[2], Zhou L[1], Luo M[1], Zeng L[1], Zhao X[1], Ma Y[2], Zhou Z[1], Sun L[3]
Affiliations
PMID: 34789158DOI: 10.1186/s12864-021-08150-8
Impact factor: 4.547
Abstract
background: GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Here, we present the first report to compare the transcriptome and LncRNA sequencing data of the GenoLab M sequencer to NovaSeq 6000 platform in various types of analysis.
results: We tested 16 libraries in three species using various library kits from different companies. We compared the data quality, genes expression, alternatively spliced (AS) events, single nucleotide polymorphism (SNP), and insertions-deletions (InDel) between two sequencing platforms. The data suggested that platforms have comparable sensitivity and accuracy in terms of quantification of gene expression levels with technical compatibility.
conclusions: Genolab M is a promising next-generation sequencing platform for transcriptomics and LncRNA studies with high performance at low costs.
Keywords: Compatibility; GenoLab M; LncRNA; NovaSeq 6000; Transcriptome
MeSH terms
Gene Library; High-Throughput Nucleotide Sequencing; INDEL Mutation; RNA, Long Noncoding; Transcriptome
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