Genome-scale deconvolution of RNA structure ensembles.
Nat Methods, 2021/03;18(3):249-252.
Morandi E[1, 2], Manfredonia I[1], Simon LM[2], Anselmi F[2], van Hemert MJ[3], Oliviero S[4, 5], Incarnato D[6]
Affiliations
PMID: 33619392DOI: 10.1038/s41592-021-01075-w
Impact factor: 47.99
Abstract
RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3' untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.
MeSH terms
3' Untranslated Regions; Algorithms; COVID-19; Genome, Viral; Humans; Mutation; Nucleic Acid Conformation; RNA, Viral; SARS-CoV-2; Sulfuric Acid Esters
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