Site-Dependent Lineage Preference of Adipose Stem Cells.
Front Cell Dev Biol, 2020;8:237.
Wang T[1, 2], Hill RC[3], Dzieciatkowska M[3], Zhu L[2], Infante AM[4], Hu G[4, 5], Hansen KC[3], Pei M[1, 6]
Affiliations
PMID: 32351957DOI: 10.3389/fcell.2020.00237
Impact factor: 6.081
Abstract
Adult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depot-dependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular discrepancy in adipose stem cells and their corresponding matrix microenvironments. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating a site-dependent lineage preference of adipose stem cells.
Keywords: adipogenesis; adipose stem cell; chondrogenesis; infrapatellar fat pad; osteogenesis; subcutaneous adipose tissue
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download