Nocardioides yefusunii sp. nov., isolated from Equus kiang (Tibetan wild ass) faeces.
Int J Syst Evol Microbiol, 2019/11;69(11):3629-3635.
Huang Y[1, 2], Wang X[1, 2], Yang J[3, 4, 1], Lu S[3, 4, 1], Lai XH[5], Jin D[3, 4, 1], Pu J[1], Huang Y[1], Ren Z[3, 1], Zhu W[4], Meng J[1, 2], Dong K[1], Ge Y[1], Zhou J[1], Zhang G[1], Zhang S[1], Lei W[1], Liang H[2], Xu J[4, 1, 2, 3]
Affiliations
PMID: 31511125DOI: 10.1099/ijsem.0.003674
Impact factor: 2.689
Abstract
Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China. Phylogenetic analyses indicated that strains HY056T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608T, Nocardioides houyundeii 78T, Nocardioides solisilvae Ka25T and Nocardioides gilvus XZ17T. The digital DNA-DNA hybridization value of strain HY056T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives. MK-8(H4) was the predominant (91.6 %) respiratory quinone. The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phospholipid. The predominant fatty acids (>10.0 %) were C18 : 1ω9c and iso-C16:0. The DNA G+C contents of strains HY056T and HY057 were 68.9 and 69.1 mol%, respectively. β-Glucosidase expression was positive, and acid was produced from d-fructose. Strain HY056T (=CGMCC 4.7563T=JCM 33399T) is assigned as the type strain of a novel species within the genus Nocardioides, for which the name Nocardioidesyefusunii sp. nov is proposed.
Keywords: Equus kiang; Nocardioides yefusunii sp. nov.; Qinghai-Tibet Plateau; Tibetan wild ass
MeSH terms
Actinobacteria; Animals; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Diaminopimelic Acid; Equidae; Fatty Acids; Feces; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Tibet; Vitamin K 2
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