Azospirillum ramasamyi sp. nov., a novel diazotrophic bacterium isolated from fermented bovine products.
Int J Syst Evol Microbiol, 2019/5;69(5):1369-1375.
Anandham R[1], Heo J[2], Krishnamoorthy R[1], SenthilKumar M[1], Gopal NO[1], Kim SJ[2], Kwon SW[2]
Affiliations
PMID: 30810523DOI: 10.1099/ijsem.0.003320
Impact factor: 2.689
Abstract
A Gram-stain-negative, rod-shaped, aerobic bacterium, designated M2T2B2T, was isolated from fermented bovine products in Suwon, Republic of Korea. The strain displayed growth at 15-45 °C (optimum, 28-30 °C), pH 6.0-10.0 (pH 7.0) and 0-2 % (w/v) NaCl (0 %). Colonies were light pink-coloured, round and convex. The cells were positive for oxidase and weakly positive for catalase. The major fatty acids in whole cells of strain M2T2B2T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), followed by summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), summed feature 2 (C12 : 0 aldehyde/unidentified 10.928/C14 : 0 3-OH/iso-C16 : 1 I), C16 : 0, C18 : 1 2-OH, C16 : 0 3-OH and C17 : 1ω6c. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and three unidentified aminolipids. Ubiquinone 10 was the predominant ubiquinone. The DNA G+C content was 68.0 mol%. The strain could fix atmospheric nitrogen, which was evaluated by the acetylene reduction assay. Further, whole genome sequence analysis revealed the presence of a nif gene cluster. Strain M2T2B2T showed the highest 16S rRNA, rpoD and nifH gene sequence similarity to members of the genus Azospirillum, and showed 97.6 % 16S rRNA gene sequence similarity to Azospirillum oryzae COC8T. The phenotypic, phylogenetic and genomic analyses support the proposal of strain M2T2B2T as being a novel species of the genus Azospirillum, for which the name Azospirillumramasamyi sp. nov. is proposed. The type strain is M2T2B2T (=KACC 14063T=NBRC 106460T).
Keywords: Alphaproteobacteria; Azospirillum ramasamyi sp. nov; diazotroph; fermented bovine products; organic fertilizer
MeSH terms
Animals; Azospirillum; Bacterial Typing Techniques; Base Composition; Cattle; Cultured Milk Products; DNA, Bacterial; Fatty Acids; Food Microbiology; Genes, Bacterial; Phospholipids; Phylogeny; Pigmentation; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Ubiquinone
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