Nucleotide sequence of the regulatory gene xylS on the Pseudomonas putida TOL plasmid and identification of the protein product.
Gene, 1986;44(2-3):235-42.
Inouye S, Nakazawa A, Nakazawa T
PMID: 3023186
Impact factor: 3.913
Abstract
The xylS gene is a regulatory gene which positively controls expression of the genes on the TOL plasmid for degradation enzymes of benzoate or m-toluate in Pseudomonas putida. Cloning of the gene in Escherichia coli and determination of the nucleotide sequence revealed an open reading frame of 963 bp which corresponds to a protein with an Mr of 36,502. The xylS gene was recloned onto a tac-promoter vector, and the product was identified by the maxicell procedure as a protein with an approximate Mr of 37,000. The predicted amino acid sequence of XylS protein showed a basic character and contained a region similar to those in other DNA-binding proteins.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; Base Sequence; Benzoates; DNA Restriction Enzymes; Escherichia coli; Genes, Bacterial; Genes, Regulator; Genotype; Plasmids; Pseudomonas
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