Exploring the sound-modulated delay in tomato ripening through expression analysis of coding and non-coding RNAs.
Ann Bot, 2018/12/31;122(7):1231-1244.
Kim JY[1], Kim SK[2], Jung J[3, 4], Jeong MJ[1], Ryu CM[3]
Affiliations
PMID: 30010774DOI: 10.1093/aob/mcy134
Impact factor: 5.04
Abstract
background and aims: Sound is omnipresent in nature. Recent evidence supports the notion that naturally occurring and artificially generated sound waves induce inter- and intracellular changes in plants. These changes, in turn, lead to diverse physiological changes, such as enhanced biotic and abiotic stress responses, in both crops and model plants.
methods: We previously observed delayed ripening in tomato fruits exposed to 1 kHz sound vibrations for 6 h. Here, we evaluated the molecular mechanism underlying this delaying fruit ripening by performing RNA-sequencing analysis of tomato fruits at 6 h, 2 d, 5 d and 7 d after 1 kHz sound vibration treatment.
key results: Bioinformatic analysis of differentially expressed genes and non-coding small RNAs revealed that some of these genes are involved in plant hormone and cell wall modification processes. Ethylene and cytokinin biosynthesis and signalling-related genes were downregulated by sound vibration treatment, whereas genes involved in flavonoid, phenylpropanoid and glucan biosynthesis were upregulated. Furthermore, we identified two sound-specific microRNAs and validated the expression of the pre-microRNAs and the mRNAs of their target genes.
conclusions: Our results indicate that sound vibration helps to delay fruit ripening through the sophisticated regulation of coding and non-coding RNAs and transcription factor genes.
MeSH terms
Fruit; Gene Expression Profiling; Gene Expression Regulation, Plant; Solanum lycopersicum; RNA, Plant; RNA, Untranslated; Sound; Time Factors; Vibration
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