Completion of genome of Aeromonas salmonicida subsp. salmonicida 01-B526 reveals how sequencing technologies can influence sequence quality and result interpretations.
New Microbes New Infect, 2018/9;25:24-26.
Vincent AT[1], Charette SJ[2, 3, 4]
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PMID: 29983988DOI: 10.1016/j.nmni.2018.05.007
Abstract
Aeromonas salmonicida subsp. salmonicida is a pathogen that primarily infects salmonids. A strain of this bacterium, 01-B526, has been used in several studies as a reference. The genomic sequence of this strain is available, but comes from pyrosequencing and is the second most fragmented assembly for this bacterium. We generated its closed genome sequence and found a pitfall in result interpretations associated with low-quality genomic sequences.
Keywords: Aeromonas salmonicida; Genomics; PacBio; average nucleotide identity; sequencing technologies
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