Molecular phylogeny and characterization of secondary metabolite profile of plant pathogenic Alternaria species isolated from basil.
Food Microbiol, 2018/8;73:264-274.
Siciliano I[1], Franco Ortega S[2], Gilardi G[1], Bosio P[3], Garibaldi A[1], Gullino ML[2]
Affiliations
PMID: 29526211DOI: 10.1016/j.fm.2018.02.001
Impact factor: 6.374
Abstract
Alternaria leaf-spot is a new disease recently reported on basil in Italy. The correct identification of Alternaria species has suffered from many reclassifications in function of morphological features and molecular data. In our study, we performed an overall approach to obtain a better characterization of basil Alternaria isolates. Morphological characteristics, seven-genome region phylogenic analysis, and secondary metabolite profile differentiated the majority of the isolates as A. alternata. OPA 1-3 and OPA 10-2 were the best molecular regions to discriminate among the isolates. Morphological characteristics and sporulation groups helped to discriminate A. tenuissima from A. alternata isolates. All isolates in the A. sect. Alternaria were mycotoxigenic and pathogenic on basil, the production of mycotoxins was enhanced on basil compared to in vitro conditions used in this work.
Keywords: Alternaria leaf-spot; HPLC-MS/MS; Morphology; Multilocus analysis; Ocimum basilicum
MeSH terms
Alternaria; Italy; Mycotoxins; Ocimum basilicum; Phylogeny; Plant Diseases; Secondary Metabolism; Spores, Fungal
More resources
EndNote: Download