Comprehensive genomic analysis of a plant growth-promoting rhizobacterium Pantoea agglomerans strain P5.
Sci Rep, 2017/11/15;7(1):15610.
Shariati J V[1, 2], Malboobi MA[3, 4], Tabrizi Z[1, 2], Tavakol E[5], Owlia P[6], Safari M[7]
Affiliations
PMID: 29142289DOI: 10.1038/s41598-017-15820-9
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Abstract
In this study, we provide a comparative genomic analysis of Pantoea agglomerans strain P5 and 10 closely related strains based on phylogenetic analyses. A next-generation shotgun strategy was implemented using the Illumina HiSeq 2500 technology followed by core- and pan-genome analysis. The genome of P. agglomerans strain P5 contains an assembly size of 5082485 bp with 55.4% G + C content. P. agglomerans consists of 2981 core and 3159 accessory genes for Coding DNA Sequences (CDSs) based on the pan-genome analysis. Strain P5 can be grouped closely with strains PG734 and 299 R using pan and core genes, respectively. All the predicted and annotated gene sequences were allocated to KEGG pathways. Accordingly, genes involved in plant growth-promoting (PGP) ability, including phosphate solubilization, IAA and siderophore production, acetoin and 2,3-butanediol synthesis and bacterial secretion, were assigned. This study provides an in-depth view of the PGP characteristics of strain P5, highlighting its potential use in agriculture as a biofertilizer.
MeSH terms
Comparative Genomic Hybridization; Gene Regulatory Networks; Genome, Bacterial; Genomics; Molecular Sequence Annotation; Pantoea; Phylogeny; Plant Diseases; Whole Genome Sequencing
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