Using two-site binding models to analyze microscale thermophoresis data.
Anal Biochem, 2018/01/01;540-541:64-75.
Tso SC[1], Chen Q[2], Vishnivetskiy SA[2], Gurevich VV[2], Iverson TM[3], Brautigam CA[4]
所属单位
PMID: 29054528DOI: 10.1016/j.ab.2017.10.013
影响因子：3.191
摘要
The emergence of microscale thermophoresis (MST) as a technique for determining the dissociation constants for bimolecular interactions has enabled these quantities to be measured in systems that were previously difficult or impracticable. However, most models for analyses of these data featured the assumption of a simple 1:1 binding interaction. The only model widely used for multiple binding sites was the Hill equation. Here, we describe two new MST analytic models that assume a 1:2 binding scheme: the first features two microscopic binding constants (KD(1) and KD(2)), while the other assumes symmetry in the bivalent molecule, culminating in a model with a single macroscopic dissociation constant (KD,M) and a single factor (α) that accounts for apparent cooperativity in the binding. We also discuss the general applicability of the Hill equation for MST data. The performances of the algorithms on both real and simulated data are assessed, and implementation of the algorithms in the MST analysis program PALMIST is discussed.
关键词：Arrestin-3; DNA aptamer; Microscale thermophoresis; Protein-ligand interactions; Protein-protein interactions
医学主题词
Adenosine Monophosphate; Algorithms; Animals; Aptamers, Nucleotide; Binding Sites; Cattle; Kinetics; Models, Molecular; Monte Carlo Method; Mutagenesis, Site-Directed; Phytic Acid; Protein Binding; Recombinant Proteins; beta-Arrestin 2
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