Highly selective on-off fluorescence recognition of Fe3+ based on a coumarin derivative and its application in live-cell imaging.
Spectrochim Acta A Mol Biomol Spectrosc, 2018/1/05;188:659-665.
Warrier S[1], Kharkar PS[2]
Affiliations
PMID: 28803123DOI: 10.1016/j.saa.2017.07.068
Impact factor: 4.831
Abstract
A novel coumarin chemosensor, 7-hydroxy-2-oxo-N-(pyridin-2-ylmethyl)chromene-3-carboxamide (Probe 1), demonstrated significant selectivity towards Fe3+ ions. Probe 1 exhibited high fluorescence emission profile at 447nm, excellent selectivity towards Fe3+ over other biologically important metal ions (Al3+, Ba2+, Co2+, Cu2+, Zn2+, Cd2+, Hg2+, Pb2+ and Sn2+). Interestingly, there was ~30-fold decrease in fluorescence intensity upon Fe3+ binding. The limit of detection of Fe3+ was found to be 0.76μM (~40ppb). Probe 1 also exhibited high potential as an intracellular chemosensor for Fe3+.
Keywords: Coumarin; Fe(3+) probes; Fluorescence quenching; Fluorescent chemosensor; Live-cell imaging
MeSH terms
Animals; Calibration; Cell Death; Cell Line; Cell Survival; Coumarins; Fluorescence; Fluorescent Dyes; Hydrogen-Ion Concentration; Imaging, Three-Dimensional; Ions; Iron; Limit of Detection; Solubility; Solvents; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet
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