Genomic confirmation of vancomycin-resistant Enterococcus transmission from deceased donor to liver transplant recipient.
PLoS One, 2017;12(3):e0170449.
Bashir A[1], Attie O[1], Sullivan M[1], Sebra R[1], Singh KV[2], Altman D[3], Pak T[1], Dutta J[1], Chacko K[1], Webster E[1], Lewis M[1], Hamula C[4], Delli Carpini KW[5], Murray BE[2], Kasarskis A[1], van Bakel H[1], Huprikar S[3]
Affiliations
PMID: 28301471DOI: 10.1371/journal.pone.0170449
Impact factor: 3.752
Abstract
In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.
MeSH terms
Aged; Female; Genes, Bacterial; Humans; Liver Transplantation; Male; Middle Aged; Tissue Donors; Vancomycin-Resistant Enterococci
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