Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant.
PLoS One, 2017;12(2):e0170715.
Omidvar V[1], Mohorianu I[2, 3], Dalmay T[3], Zheng Y[4], Fei Z[4], Pucci A[5], Mazzucato A[5], Večeřová V[6], Sedlářova M[6], Fellner M[1]
Affiliations
PMID: 28178307DOI: 10.1371/journal.pone.0170715
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Abstract
The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 7B-1, transcriptomic profiles of the 7B-1 male-sterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.
MeSH terms
Computational Biology; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Ontology; Glucans; Solanum lycopersicum; Mutation; Plant Infertility; Transcription, Genetic
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