Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation.
PLoS One, 2016;11(8):e0159723.
Song B[1], An L[2], Han Y[1], Gao H[1], Ren H[3], Zhao X[1], Wei X[1], Krishnan HB[4], Liu S[1]
Affiliations
PMID: 27532666DOI: 10.1371/journal.pone.0159723
Impact factor: 3.752
Abstract
Crossing, backcrossing, and molecular marker-assisted background selection produced a soybean (Glycine max) near-isogenic line (cgy-2-NIL) containing the cgy-2 allele, which is responsible for the absence of the allergenic α-subunit of β-conglycinin. To identify α-null-related transcriptional changes, the gene expressions of cgy-2-NIL and its recurrent parent DN47 were compared using Illumina high-throughput RNA-sequencing of samples at 25, 35, 50, and 55 days after flowering (DAF). Seeds at 18 DAF served as the control. Comparison of the transcript profiles identified 3,543 differentially expressed genes (DEGs) between the two genotypes, with 2,193 genes downregulated and 1,350 genes upregulated. The largest numbers of DEGs were identified at 55 DAF. The DEGs identified at 25 DAF represented a unique pattern of GO category distributions. KEGG pathway analyses identified 541 altered metabolic pathways in cgy-2-NIL. At 18DAF, 12 DEGs were involved in arginine and proline metabolism. The cgy-2 allele in the homozygous form modified the expression of several Cupin allergen genes. The cgy-2 allele is an alteration of a functional allele that is closely related to soybean protein amino acid quality, and is useful for hypoallergenic soybean breeding programs that aim to improve seed protein quality.
MeSH terms
Antigens, Plant; Base Sequence; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Globulins; High-Throughput Nucleotide Sequencing; Plant Breeding; Seed Storage Proteins; Seeds; Sequence Analysis, RNA; Soybean Proteins; Glycine max; Transcriptome
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download