Deep sequencing leads to the identification of eukaryotic translation initiation factor 5A as a key element in Rsv1-mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean.
Mol Plant Pathol, 2017/04;18(3):391-404.
Chen H[1, 2], Adam Arsovski A[1], Yu K[3], Wang A[1, 2]
Affiliations
PMID: 27019403DOI: 10.1111/mpp.12407
Impact factor: 5.52
Abstract
Rsv1, a single dominant resistance locus in soybean, confers extreme resistance to the majority of Soybean mosaic virus (SMV) strains, but is susceptible to the G7 strain. In Rsv1-genotype soybean, G7 infection provokes a lethal systemic hypersensitive response (LSHR), a delayed host defence response. The Rsv1-mediated LSHR signalling pathway remains largely unknown. In this study, we employed a genome-wide investigation to gain an insight into the molecular interplay between SMV G7 and Rsv1-genotype soybean. Small RNA (sRNA), degradome and transcriptome sequencing analyses were used to identify differentially expressed genes (DEGs) and microRNAs (DEMs) in response to G7 infection. A number of DEGs, DEMs and microRNA targets, and the interaction network of DEMs and their target mRNAs responsive to G7 infection, were identified. Knock-down of one of the identified DEGs, the eukaryotic translation initiation factor 5A (eIF5A), diminished the LSHR and enhanced viral accumulation, suggesting the essential role of eIF5A in the G7-induced, Rsv1-mediated LSHR signalling pathway. This work provides an in-depth genome-wide analysis of high-throughput sequencing data, and identifies multiple genes and microRNA signatures that are associated with the Rsv1-mediated LSHR.
Keywords: Soybean mosaic virus; degradome-seq; eukaryotic translation initiation factor; lethal systemic hypersensitive response; sRNA-seq; transcriptome-seq; virus-host interaction
MeSH terms
Databases, Genetic; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Genotype; High-Throughput Nucleotide Sequencing; MicroRNAs; Peptide Initiation Factors; Plant Diseases; Plant Proteins; Potyvirus; RNA Stability; RNA-Binding Proteins; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Glycine max; Transcription, Genetic; Eukaryotic Translation Initiation Factor 5A
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