Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis.
Sci Rep, 2016/3/15;6:22896.
Lee SH[1, 2], Kim MS[3], Lee JH[1], Kim TW[1, 2], Bae SS[1], Lee SM[1], Jung HC[1, 2], Yang TJ[1], Choi AR[1], Cho YJ[4], Lee JH[1, 2], Kwon KK[1, 2], Lee HS[1, 2], Kang SG[1, 2]
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PMID: 26975345DOI: 10.1038/srep22896
Impact factor: 4.996
Abstract
The hyperthermophilic archaeon Thermococcus onnurineus NA1 can grow and produce H2 on carbon monoxide (CO) and its H2 production rates have been improved through metabolic engineering. In this study, we applied adaptive evolution to enhance H2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H2 productivity at high CO flow rates using synthesis gas produced from coal gasification.
MeSH terms
Adaptation, Physiological; Carbon Monoxide; DNA Methylation; Energy Metabolism; Epigenomics; Gene Expression Profiling; Genes, Archaeal; Genome, Archaeal; Genomics; Hot Temperature; Hydrogen; Metabolic Engineering; Mutation; Oxidation-Reduction; Thermococcus
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