Transcriptome analysis of Streptococcus pneumoniae D39 in the presence of cobalt.
Genom Data, 2015/12;6:151-3.
Manzoor I[1], Shafeeq S[2], Kuipers OP[3]
Affiliations
PMID: 26697359DOI: 10.1016/j.gdata.2015.08.033
Abstract
Cobalt (Co(2 +)) is an important transition metal ion that plays a vital role in cellular physiology of bacteria. The role of Co(2 +) in the regulation of several genes/operons in Streptococcus pneumoniae has recently been reported [1]. The data described in this article relate to the genome-wide transcriptional profiling of Streptococcus pneumoniae D39, either in the presence or absence of 0.5 mM Co(2 +) in chemically defined medium (CDM) using DNA microarray analysis. Genes belonging to a broad range of cellular processes such as virulence, transport and efflux systems, stress response and surface attachment were differentially expressed in the presence of Co(2 +). We used transcriptional lacZ assays and electrophoretic mobility shift assays (EMSAs) to confirm our results [1]. The dataset is publicly available at the Gene Expression Omnibus (GEO) repository (http://www.ncbi.nlm.nih.gov/geo/) with accession number GSE57696.
Keywords: Co2 +; Microarray; PsaR; Streptococcus pneumoniae
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