The mystery of the fourth clone: comparative genomic analysis of four non-typeable Streptococcus pneumoniae strains with different susceptibilities to optochin.
Eur J Clin Microbiol Infect Dis, 2016/1;35(1):119-30.
Ikryannikova LN[1], Ischenko DS[2], Lominadze GG[3], Kanygina AV[2], Karpova IY[2], Kostryukova ES[2], Mayansky NA[3], Skvortsov VS[4], Ilina EN[2], Govorun VM[2]
Affiliations
PMID: 26563895DOI: 10.1007/s10096-015-2516-5
Impact factor: 5.103
Abstract
Optochin-resistant pneumococci can be rarely caught in clinical microbiology laboratories because of the routine identification of all such strains as viridans group non-pneumococci. We were lucky to find four non-typeable Streptococcus pneumoniae clones demonstrating the different susceptibilities to optochin: one of them (Spn_13856) was resistant to optochin, while the other three (Spn_1719, Spn_27, and Spn_2298) were susceptible. Whole genome nucleotide sequences of these strains were compared to reveal the differences between the optochin-resistant and optochin-susceptible strains. Two adjacent genes coding maltose O-acetyltransferase and uridine phosphorylase which were presented in the genomes of all optochin-susceptible strains and missed in the optochin-resistant strain were revealed. Non-synonymous substitutions in 14 protein-coding genes were discovered, including the Ala49Ser mutation in the C-subunit of the F0 part of the ATP synthase rotor usually associated with pneumococcal optochin resistance. Modeling of a process of optochin interaction with the F0 part of the ATP synthase rotor indicates that the complex of optochin with "domain C" composed by wild-type C-subunits is more stable than the same complex composed of Ala49Ser mutant C-subunits.
MeSH terms
Anti-Infective Agents; Drug Resistance, Bacterial; Genome, Bacterial; Genomics; Humans; Microbial Sensitivity Tests; Mitochondrial Proton-Translocating ATPases; Molecular Dynamics Simulation; Mutation, Missense; Pneumococcal Infections; Protein Binding; Quinine; Sequence Analysis, DNA; Streptococcus pneumoniae
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