GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae.
J Mol Microbiol Biotechnol, 2015;25(6):363-71.
Afzal M[1], Shafeeq S, Manzoor I, Kuipers OP
Affiliations
PMID: 26544195DOI: 10.1159/000439429
Abstract
We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3') for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of D39 ΔgalK with the D39 wild type grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose.
MeSH terms
Bacterial Proteins; Base Sequence; DNA, Bacterial; Escherichia coli Proteins; Galactokinase; Galactose; Genes, Bacterial; Glucose; Hexoses; Microarray Analysis; Molecular Sequence Data; Mutation; Operon; Promoter Regions, Genetic; Real-Time Polymerase Chain Reaction; Repressor Proteins; Streptococcus pneumoniae; Transcriptional Activation; UTP-Hexose-1-Phosphate Uridylyltransferase
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