Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825* and activating defense-related genes in Arabidopsis.
J Integr Plant Biol, 2016/4;58(4):426-39.
Niu D[1, 2], Xia J[3, 4], Jiang C[1], Qi B[1], Ling X[1], Lin S[1], Zhang W[3], Guo J[1], Jin H[2], Zhao H[1]
Affiliations
PMID: 26526683DOI: 10.1111/jipb.12446
Impact factor: 9.106
Abstract
Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance (ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here, by comparing small RNA profiles of Pseudomonas syringae pv. tomato (Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis microRNAs (miRNAs) that are differentially regulated by AR156 pretreatment. miR825 and miR825* are two miRNA generated from a single miRNA gene. Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. miR825 targets two ubiquitin-protein ligases, while miR825* targets toll-interleukin-like receptor (TIR)-nucleotide binding site (NBS) and leucine-rich repeat (LRR) type resistance (R) genes. The expression of these target genes negatively correlated with the expression of miR825 and miR825*. Moreover, transgenic plants showing reduced expression of miR825 and miR825* displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing miR825 and miR825* were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing miR825 and miR825* and activating the defense related genes they targeted.
Keywords: ISR; Induced systemic resistance; microRNA; plant innate immunity; small RNA
MeSH terms
Arabidopsis; Bacillus cereus; Base Sequence; Disease Resistance; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Knockdown Techniques; Genes, Plant; Genetic Loci; MicroRNAs; Plant Diseases; Plants, Genetically Modified; Pseudomonas syringae; RNA, Small Interfering
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