Identification of the centromeric repeat in the threespine stickleback fish (Gasterosteus aculeatus).
Chromosome Res, 2015/12;23(4):767-79.
Cech JN[1, 2], Peichel CL[3]
Affiliations
PMID: 26424612DOI: 10.1007/s10577-015-9495-3
Impact factor: 4.62
Abstract
Centromere sequences exist as gaps in many genome assemblies due to their repetitive nature. Here we take an unbiased approach utilizing centromere protein A (CENP-A) chomatin immunoprecipitation followed by high-throughput sequencing to identify the centromeric repeat sequence in the threespine stickleback fish (Gasterosteus aculeatus). A 186-bp, AT-rich repeat was validated as centromeric using both fluorescence in situ hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This repeat hybridizes strongly to the centromere on all chromosomes, with the exception of weak hybridization to the Y chromosome. Together, our work provides the first validated sequence information for the threespine stickleback centromere.
Keywords: CENP-A; Centromere; ChIP-seq; Gasterosteus aculeatus; Threespine stickleback
MeSH terms
Animals; Autoantigens; Base Sequence; Binding Sites; Centromere; Centromere Protein A; Chromosomal Proteins, Non-Histone; Female; Fishes; In Situ Hybridization, Fluorescence; Male; Molecular Sequence Data; Open Reading Frames; Protein Binding; Sequence Alignment; Tandem Repeat Sequences; Y Chromosome
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