Cathepsin K Contributes to Cavitation and Collagen Turnover in Pulmonary Tuberculosis.
J Infect Dis, 2016/2/15;213(4):618-27.
Kubler A[1], Larsson C[2], Luna B[3], Andrade BB[4], Amaral EP[4], Urbanowski M[3], Orandle M[5], Bock K[5], Ammerman NC[3], Cheung LS[3], Winglee K[3], Halushka M[6], Park JK[7], Sher A[4], Friedland JS[8], Elkington PT[9], Bishai WR[3]
Affiliations
PMID: 26416658DOI: 10.1093/infdis/jiv458
Impact factor: 7.759
Abstract
Cavitation in tuberculosis enables highly efficient person-to-person aerosol transmission. We performed transcriptomics in the rabbit cavitary tuberculosis model. Among 17 318 transcripts, we identified 22 upregulated proteases. Five type I collagenases were overrepresented: cathepsin K (CTSK), mast cell chymase-1 (CMA1), matrix metalloproteinase 1 (MMP-1), MMP-13, and MMP-14. Studies of collagen turnover markers, specifically, collagen type I C-terminal propeptide (CICP), urinary deoxypyridinoline (DPD), and urinary helical peptide, revealed that cavitation in tuberculosis leads to both type I collagen destruction and synthesis and that proteases other than MMP-1, MMP-13, and MMP-14 are involved, suggesting a key role for CTSK. We confirmed the importance of CTSK upregulation in human lung specimens, using immunohistochemical analysis, which revealed perigranulomatous staining for CTSK, and we showed that CTSK levels were increased in the serum of patients with tuberculosis, compared with those in controls (3.3 vs 0.3 ng/mL; P = .005).
Keywords: RNAseq; cathepsin K; collagen; collagenolysis; rabbit; tuberculosis
MeSH terms
Animals; Cathepsin K; Collagen; Collagenases; Disease Models, Animal; Female; Gene Expression Profiling; Immunohistochemistry; Lung; Rabbits; Tuberculosis, Pulmonary
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