Basic characterization of avian β-defensin genes in the Japanese quail, Coturnix japonica.
Anim Sci J, 2016/3;87(3):311-20.
Ishige T[1], Hara H[2], Hirano T[2], Mannen H[3], Kono T[1, 4], Hanzawa K[2]
Affiliations
PMID: 26338292DOI: 10.1111/asj.12436
Impact factor: 1.974
Abstract
In this study, we identified a cluster of 14 avian β-defensins (AvBD; approximately 66 kbp) in the Japanese quail, Coturnix japonica. Except for AvBD12 (CjAvBD12) and -13, the CjAvBDs coding sequences exhibited greater than 78.0% similarity to the respective orthologous chicken AvBD genes (GgAvBD). The putative amino acid sequence encoded by each CjAvBD contained six cysteine residues and the GXC (X1-2) motif considered essential for the β-defensin family. Each CjAvBDs also formed a sub-group with the respective orthologous genes of various bird species in a phylogenetic tree analysis. Synteny between the CjAvBD cluster and GgAvBD cluster was confirmed. The CjAvBD cluster was mapped on the long-arm end of chromosome 3 by linkage analysis based on single nucleotide polymorphisms (SNPs) of CjAvBD1 and CjAvBD12 (approximately 46 kbp), as well as GgAvBD cluster. We also confirmed that CjAvBD1, -4, -5, -9, and -10 are transcribed in 20 tissues, including immune and digestive tissues. However, our experimental data indicated that the CjAvBD cluster lacks the AvBD3 and -7 loci, whereas the CjAvBD101α, -101β, and -101θ loci arose from gene duplication of the AvBD6 orthologous locus in the CjAvBD cluster after differentiation between Coturnix - Gallus.
Keywords: Japanese quail; avian β-defensin (AvBD); linkage map; phylogenetic analysis
MeSH terms
Amino Acid Motifs; Amino Acid Sequence; Animals; Chickens; Chromosomes, Human, Pair 3; Coturnix; Cysteine; Gene Duplication; Genetic Linkage; Humans; Male; Phylogeny; Polymorphism, Single Nucleotide; beta-Defensins
More resources
EndNote: Download