Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of Stenotrophomonas maltophilia bacteremia.
Antimicrob Agents Chemother, 2015/11;59(11):7117-20.
Pak TR[1], Altman DR[2], Attie O[1], Sebra R[1], Hamula CL[3], Lewis M[1], Deikus G[1], Newman LC[1], Fang G[1], Hand J[2], Patel G[2], Wallach F[2], Schadt EE[1], Huprikar S[2], van Bakel H[1], Kasarskis A[4], Bashir A[1]
Affiliations
PMID: 26324280DOI: 10.1128/AAC.01723-15
Impact factor: 5.938
Abstract
Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.
MeSH terms
DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Genome, Bacterial; Gram-Negative Bacterial Infections; Microbial Sensitivity Tests; Mutation; Quinolones; Stenotrophomonas maltophilia
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