Nucleotide sequence of cloned nad4 (urf4) gene from Chlamydomonas reinhardtii mitochondrial DNA.
Gene, 1989/12/28;85(2):363-70.
Ma DP[1], Yang YW, King YT, Hasnain SE
Affiliations
PMID: 2628173
Impact factor: 3.913
Abstract
Chlamydomonas reinhardtii mitochondrial (mt)DNA was digested with ClaI + HpaI and shotgun cloned into the M13mp19 vector cleaved with AccI + SmaI. One of the recombinant clones, with a 1.8-kb DNA insert, was completely sequenced using the dideoxy chain-termination method. Besides containing part of the cytochrome b (COB)-encoding gene (cob), this DNA fragment encodes subunit 4 of NADH dehydrogenase (NAD4). The deduced amino acid sequence and hydrophilicity plot indicate that NAD4 is highly hydrophobic. The nad4 gene shows a unique preference for certain codons which are also found in other C. reinhardtii mt proteins. Both the genes encoding NAD4 and COB are shown to be transcriptionally active by Northern hybridization. These closely linked genes suggest that RNA-processing events found in vertebrate mt are present in Chlamydomonas mt as well.
MeSH terms
Amino Acid Sequence; Animals; Base Sequence; Chlamydomonas; Cloning, Molecular; Codon; Cytochrome Reductases; DNA, Mitochondrial; Genes, Fungal; Humans; Macromolecular Substances; Molecular Sequence Data; NADH Dehydrogenase; RNA, Fungal; Restriction Mapping; Sequence Homology, Nucleic Acid; Transcription, Genetic
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