Effects of a Tricaprylin Emulsion on Anti-glomerular Basement Membrane Glomerulonephritis in Rats: In Vivo and in Silico Studies.
Biol Pharm Bull, 2015;38(8):1175-84.
Liu N[1], Shi J, Xiao Y, Yasue M, Takei Y, Sanefuji H, Tsujimoto G, Hirasawa A
Affiliations
PMID: 26235580DOI: 10.1248/bpb.b15-00124
Impact factor: 2.264
Abstract
Glomerulonephritis (GN) is a set of pathological conditions that result in the destruction of glomeruli and loss of renal function, commonly leading to the development of end-stage renal disease. Current pharmacotherapy is limited to immunosuppressive therapy. In the present study, we found a novel antinephritic effect of a tricaprylin emulsion in the anti-glomerular basement membrane (anti-GBM) GN rat model. We evaluated the treatment in vivo by comparing administration of the emulsion with administration of a casein kinase II (CK2) inhibitor in this rat model, and performed a gene ontology-based microarray analysis to reveal in silico the detailed mechanism of action. Our results showed that administration of the tricaprylin emulsion, or even tricaprylin alone, significantly ameliorated the anti-GBM antibody-induced renal dysfunction in these rats. We believe that tricaprylin is the key active antinephritic component of the emulsion and might be a promising drug for the effective treatment of nephritis. Moreover, with respect to microarray analysis, we developed a generally applicable and rapid method to compare gene expression profile data for multiple models of nephritis and clinical samples from a public domain microarray database.
MeSH terms
Animals; Anti-Glomerular Basement Membrane Disease; Autoantibodies; Caprylates; Computer Simulation; Emulsions; Glomerular Basement Membrane; Glomerulonephritis; Kidney; Kidney Failure, Chronic; Male; Microarray Analysis; Rats, Inbred Strains; Transcriptome; Triglycerides
More resources
EndNote: Download