Sialic acid-mediated gene expression in Streptococcus pneumoniae and role of NanR as a transcriptional activator of the nan gene cluster.
Appl Environ Microbiol, 2015/5/01;81(9):3121-31.
Afzal M[1], Shafeeq S[2], Ahmed H[3], Kuipers OP[4]
Affiliations
PMID: 25724955DOI: 10.1128/AEM.00499-15
Impact factor: 5.005
Abstract
In this study, we investigated the transcriptomic response of Streptococcus pneumoniae D39 to sialic acid (N-acetylneuraminic acid [Neu5Ac]). Transcriptome comparison of wild-type D39 grown in M17 medium with and without sialic acid revealed the elevated expression of various genes and operons, including the nan gene cluster (nan operon I and nanA gene). Our microarray analysis and promoter-lacZ fusion studies showed that the transcriptional regulator NanR acts as a transcriptional activator of nan operon I and the nanA gene in the presence of sialic acid. The putative regulatory site of NanR in the promoter region of nan operon I is predicted and confirmed by promoter truncation experiments. Furthermore, the role of CcpA in the regulation of the nan gene cluster is demonstrated through microarray analysis and promoter-lacZ fusion studies, suggesting that in the presence of sialic acid and glucose, CcpA represses the expression of nan operon I while the expression of the nanA gene is CcpA independent.
MeSH terms
Culture Media; DNA, Bacterial; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Glucose; Microarray Analysis; Molecular Sequence Data; Multigene Family; N-Acetylneuraminic Acid; Sequence Analysis, DNA; Streptococcus pneumoniae; Trans-Activators
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