Regulated genes in mesenchymal stem cells and gastric cancer.
World J Stem Cells, 2015/1/26;7(1):208-22.
Tanabe S[1], Aoyagi K[1], Yokozaki H[1], Sasaki H[1]
Affiliations
PMID: 25621121DOI: 10.4252/wjsc.v7.i1.208
Impact factor: 5.247
Abstract
aim: To investigate the genes regulated in mesenchymal stem cells (MSCs) and diffuse-type gastric cancer (GC), gene expression was analyzed.
methods: Gene expression of MSCs and diffuse-type GC cells were analyzed by microarray. Genes related to stem cells, cancer and the epithelial-mesenchymal transition (EMT) were extracted from human gene lists using Gene Ontology and reference information. Gene panels were generated, and messenger RNA gene expression in MSCs and diffuse-type GC cells was analyzed. Cluster analysis was performed using the NCSS software.
results: The gene expression of regulator of G-protein signaling 1 (RGS1) was up-regulated in diffuse-type GC cells compared with MSCs. A panel of stem-cell related genes and genes involved in cancer or the EMT were examined. Stem-cell related genes, such as growth arrest-specific 6, musashi RNA-binding protein 2 and hairy and enhancer of split 1 (Drosophila), NOTCH family genes and Notch ligands, such as delta-like 1 (Drosophila) and Jagged 2, were regulated.
conclusion: Expression of RGS1 is up-regulated, and genes related to stem cells and NOTCH signaling are altered in diffuse-type GC compared with MSCs.
Keywords: Epithelial-mesenchymal transition; Gastric cancer; Gene; Mesenchymal stem cells; Stem cells
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