Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing.
Nat Methods, 2015/3;12(3):230-2, 1 p following 232.
Ziller MJ[1], Hansen KD[2], Meissner A[1], Aryee MJ[3]
Affiliations
PMID: 25362363DOI: 10.1038/nmeth.3152
Impact factor: 47.99
Abstract
Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates.
MeSH terms
Brain; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; CpG Islands; DNA Methylation; Data Interpretation, Statistical; Databases, Genetic; Embryonic Stem Cells; Genome, Human; High-Throughput Nucleotide Sequencing; Humans; Sensitivity and Specificity; Sequence Analysis, DNA; Sulfites
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download