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25314618
Comparative functional genomics of adaptation to muscular disuse in hibernating mammals.

Mol Ecol, 2014/11;23(22):5524-37.

Fedorov VB[1], Goropashnaya AV, Stewart NC, Tøien Ø, Chang C, Wang H, Yan J, Showe LC, Showe MK, Barnes BM

Affiliations

PMID: 25314618DOI: 10.1111/mec.12963

Impact factor: 6.622

Abstract
Hibernation is an energy-saving adaptation that involves a profound suppression of physical activity that can continue for 6-8 months in highly seasonal environments. While immobility and disuse generate muscle loss in most mammalian species, in contrast, hibernating bears and ground squirrels demonstrate limited muscle atrophy over the prolonged periods of physical inactivity during winter, suggesting that hibernating mammals have adaptive mechanisms to prevent disuse muscle atrophy. To identify common transcriptional programmes that underlie molecular mechanisms preventing muscle loss, we conducted a large-scale gene expression screen in hind limb muscles comparing hibernating and summer-active black bears and arctic ground squirrels using custom 9600 probe cDNA microarrays. A molecular pathway analysis showed an elevated proportion of overexpressed genes involved in all stages of protein biosynthesis and ribosome biogenesis in muscle of both species during torpor of hibernation that suggests induction of translation at different hibernation states. The induction of protein biosynthesis probably contributes to attenuation of disuse muscle atrophy through the prolonged periods of immobility of hibernation. The lack of directional changes in genes of protein catabolic pathways does not support the importance of metabolic suppression for preserving muscle mass during winter. Coordinated reduction in multiple genes involved in oxidation-reduction and glucose metabolism detected in both species is consistent with metabolic suppression and lower energy demand in skeletal muscle during inactivity of hibernation.

Keywords: arctic ground squirrel; black bear; functional genomics; gene expression; hibernation; protein biosynthesis

MeSH terms
Adaptation, Physiological; Animals; Comparative Genomic Hybridization; Hibernation; Male; Muscular Atrophy; Oligonucleotide Array Sequence Analysis; Protein Biosynthesis; Sciuridae; Transcriptome; Ursidae
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