Molecular analysis of OXA-48-carrying conjugative IncL/M-like plasmids in clinical isolates of Klebsiella pneumoniae in Ireland.
Microb Drug Resist, 2014/8;20(4):270-4.
Power K[1], Wang J, Karczmarczyk M, Crowley B, Cotter M, Haughton P, Lynch M, Schaffer K, Fanning S
Affiliations
PMID: 24520822DOI: 10.1089/mdr.2013.0022
Impact factor: 2.706
Abstract
This study characterized an IncL/M-like plasmid containing a bla(OXA-48)-encoding gene from a clinical isolate of Klebsiella pneumoniae, denoted as E71T. Investigation of this plasmid sequence identified unique regions of interest along with conserved regions detected in eight other clinical carbapenem-resistant isolates. A 63-kb plasmid (pE71T) from K. pneumoniae E71T was sequenced and found to be highly similar to the recently published K. pneumoniae pOXA-48a (JN626286). Two copies of the insertion sequence element IS1R were identified, one of which was located adjacent to the bla(OXA-48)-encoding gene forming part of a composite transposon Tn1999.2 and the second located 16-kb downstream. Plasmid profiling and PCR assays confirmed that the pE71T backbone was conserved among the eight other clinical bla(OXA-48)-positive isolates, and in all cases, the OXA-48 genes were part of the Tn1999.2 composite transposon. This is the first report of a bla(OXA-48) and IS1R arrangement-containing plasmid in Ireland.
MeSH terms
Anti-Bacterial Agents; Base Sequence; Carbapenems; Conserved Sequence; DNA Transposable Elements; Gene Expression; Humans; Ireland; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Molecular Sequence Data; Plasmids; beta-Lactam Resistance; beta-Lactamases
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