Taxonomic and phenotypic characterization of yeasts isolated from worldwide cold rock-associated habitats.
Fungal Biol, 2014/1;118(1):61-71.
Selbmann L[1], Zucconi L[1], Onofri S[1], Cecchini C[1], Isola D[1], Turchetti B[2], Buzzini P[3]
Affiliations
PMID: 24433677
Impact factor: 2.91
Abstract
Yeast strains isolated from rock samples collected from worldwide cold regions were identified by sequence analysis of the D1/D2 domains of the 26S rDNA gene and the ITS region followed by molecular phylogeny. Over 77% of yeasts isolates were Basidiomycota. Cryptococcus (orders Filobasidiales and Tremellales) and Rhodotorula (order Cystobasidiales) were the most frequent genera. About 40% of yeast isolates belonged to undescribed species. Almost all isolates were psychrotolerant. Urease and esterase were the most widely extracellular enzymatic activity at 4 °C and 20 °C. None of the strains exhibited extracellular protease, DNAse, cellulase, chitinase, and laccase activity. The taxonomic and ecological significance of yeasts associated to worldwide cold rocky habitats is discussed.
Keywords: D1/D2; Extreme environments; ITS; Lithic habitats; Phylogeny
MeSH terms
Biodiversity; Cluster Analysis; DNA, Fungal; DNA, Ribosomal; DNA, Ribosomal Spacer; Environmental Microbiology; Genes, rRNA; Molecular Sequence Data; Phylogeny; RNA, Fungal; RNA, Ribosomal; Sequence Analysis, DNA; Yeasts
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