Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect.
Eur J Nutr, 2014/4;53(3):897-906.
Denninghoff V[1], Ossani G, Uceda A, Rugnone M, Fernández E, Fresno C, González G, Díaz ML, Avagnina A, Elsner B, Monserrat A
Affiliations
PMID: 24129499DOI: 10.1007/s00394-013-0593-8
Impact factor: 4.865
Abstract
purpose: The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model.
methods: Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed.
results: All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats.
conclusions: This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.
MeSH terms
Acute Kidney Injury; Animals; Biomarkers; Choline; Choline Deficiency; Dietary Supplements; Fish Oils; Gene Expression Profiling; Glutathione S-Transferase pi; Kidney; Male; Necrosis; Oligonucleotide Array Sequence Analysis; RNA; Rats, Wistar; Transcriptome; Up-Regulation; Weaning
More resources
EndNote: Download