Molecular characterization of a novel botulinum neurotoxin type H gene.
J Infect Dis, 2014/1/15;209(2):192-202.
Dover N[1], Barash JR, Hill KK, Xie G, Arnon SS
Affiliations
PMID: 24106295DOI: 10.1093/infdis/jit450
Impact factor: 7.759
Abstract
We sequenced the 2 botulinum toxin gene clusters of Clostridium botulinum strain IBCA10-7060 type Bh. The sequence of bont/H differed substantially from the sequences of the 7 known bont genes for toxin types A-G. The 5' one-third terminus of bont/H that codes for the botulinum toxin light chain differed markedly from the light chain coding sequences of toxin types A-G. The 3' two-thirds terminus of bont/H that codes for the botulinum toxin heavy chain contained a novel Hn translocation domain coding sequence and a nonneutralizing type A-like Hc binding domain coding sequence. bont/H was part of an orfX toxin gene cluster that was located at a unique chromosomal site distant from those used by other botulinum toxin gene clusters. The bont/B sequence was similar to that of subtype bont/B2 and was located within its ha toxin gene cluster at the oppA/brnQ site. Our findings further establish that C. botulinum IBCA10-7060 produces novel BoNT/H.
Keywords: Clostridium botulinum; botulinum toxin; botulinum toxin type H; botulism
MeSH terms
Botulinum Toxins; Botulism; Clostridium botulinum; Cluster Analysis; DNA, Bacterial; Gene Order; Humans; Infant; Multigene Family; Phylogeny; Protein Subunits; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid
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