Nucleotide sequence and distribution of the pTR2030 resistance determinant (hsp) which aborts bacteriophage infection in lactococci.
Appl Environ Microbiol, 1990/7;56(7):2255-8.
Hill C[1], Miller LA, Klaenhammer TR
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PMID: 2389939
Impact factor: 5.005
Abstract
The lactococcal plasmid pTR2030 encodes resistance to bacteriophage attack via two mechanisms, an abortive-infection mechanism, designated Hsp, and a restriction and modification system. We present the complete sequence of the hsp structural gene. The gene is 1,887 base pairs in length and encodes a protein with a predicted molecular mass of 73.8 kilodaltons. The upstream region was cloned in a promoter-screening vector and shown to direct the constitutive expression of the cat-86 gene. An internal probe was used to determine the distribution of the hsp sequence in industrially significant lactococcal strains and to evaluate its relatedness to another lactococcal plasmid implicated in an abortive-infection-type mechanism, pNP40. No homology was detected, suggesting that this gene is not widely distributed in lactococci. Therefore, there are at least two independent abortive-infection genotypes in lactococci.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; Bacteriophages; Base Sequence; DNA, Bacterial; Genes, Bacterial; Lactobacillus; Molecular Sequence Data; Plasmids
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